Studi Geseran Spektrum UV
Senyawa Asam Barbiturat Pada Plat Al-TLC Si G 60 F254 Akibat Pengaruh Perbedaan
pH Pengeluen Untuk Keperluan Uji Konfirmasi
I. M. A. G. Wirasutaa,b), N.
G. Indriyaningsihb), dan N. M. Suaniti b,c)
a)Jurusan Farmasi FMIPA Universitas Udayana, Bukit
Jimbaran
b)Lembaga Forensik Sains dan Kriminologi Universitas Udayana,
Email:mgelgel1@yahoo.de
c)Jurusan Kimia FMIPA
Universitas Udayana, Bukit Jimbaran
ABSTRACT
The UV-spectra-shifting of allobarbital, barbital, butalbital and phenobarbital
on AL-TLC-Si gel 60 F254 under exposed different pH eluent, drying TLC-plat
at 110oC, and then plat placed at room temperature for more then 17
hours has been carried out. Spectra UV were read by using TLC-Scanner 3 –Camag-Muttenz-Switzerland
between 190 and 300 nm. Aim of this
research was to study UV-spectra-shifting which can conduct error by confirmation
test using in situ UV spectra.
There was no significant UV-spectra-shifting of barbiturate
on TLC-plate after exposed by mobile phase: 10% HCl in methanol (pH=1),
chloroform-acetone (80:20, v/v, pH=5), methanol-n-buthanol (60:40, v/v, pH=6),
methanol-ammonia (100:1,5, v/v, pH=10) respectively. The significant shifting
of UV spectra were obtained after TLC-plate exposed by cyclohexane-toluene-diethyl
amine (75:15:10, v/v, pH=11) and 0,1 M KOH in methanol (pH=12), the spectrum
shifting was 66% and 23% compared with spectra at pH=1. Drying of the
TLC-plates after exposed by mobile phase conducted no significant UV-spectra
shifting, except after exposed by cyclohexane-toluene-diethyl amine (the
shifting was 45%). Humidity absorption
while placing the TLC-plates at room temperature for more then 17 hours after
exposed by strong pH base lead the shifting UV-spectra of barbiturate to the
weak base or neutral spectra. These shifting UV-spectra should be taken in
consideration on confirmation test of barbiturate based TLC-UV in-situ spectra
to reduce error.
Keyword : allobarbital, barbital, butalbital,
phenobarbital, AL-TLC-Si gel 60 F254, UV spectrum
Artikel lengekap dapat di:http://ejournal.unud.ac.id/abstrak/2%281%29-1-4.pdf
